Assume you have three elution fractions, each with a volume of 500 uL. The concentration (and total amount) of GST::EGFP in the elution fraction is as follows:
Elution 1: 0.1 ug/mL (0.05 ug)
Elution 2: 0.4 ug/mL (0.2 ug)
Elution 3: 0.5 ug/mL (0.25 ug)
If the elutions were combined together, how much total GST::EGFP would be present?