. Freshly prepared mitochondria were incubated with beta-hyroxybutyrate, oxidized cytochrome c , ADP, Pi, and cyanide. Beta-hyroxybutyrate is oxidized by an NAD+ dependent dehydrogenase.

The experiment measured the rate of oxidative of beta-hyroxybutyrate and the rate of formation of ATP. a) Indicate the probable flow of electrons in this system. b) How many moles of ATP would you expect to be formed per mole of beta-hyroxybutyrate oxidized in this system? c) why is beta-hyroxybutyrate added rather than NADH? d) what is the function of the cyanide? e) write a balanced equation for the overall reaction occuring in this system ( electron transport and ATP synthesis) f) Calculate the net standart free energy charge (delta G) in this system, using Enot values from table 14.1 and a deltaG value for ATP hydrolysis of -32.2 kj/mol.